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Altogether, these data suggest that the Akt/GSK3β and NF-κB pathways are involved both in the regulation of CBS activity (as assessed by the decreased H 2 S production 24 h after treatment), but also in the regulation of CBS expression, effect which is observed at the mRNA level 24 h after treatment and at the protein level 48 h after treatment

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Treatment with 1.5 µM 2012 in SK-MEL-147 cells reduced the number of FAK-containing FAs compared to the DMSO (vehicle) treated group and an accumulation of FAK in the cytoplasm was observed (Fig